《植物生理学报》 2009, 45(8): 771-774

甜高粱离体再生体系的建立和组织结构变化的观察

徐丹, 陈立余, 徐子勤*

西北大学生命科学学院, 西部资源与现代生物技术省部共建教育部重点实验室, 陕西省生物技术重点实验室, 西安710069

通信作者：徐子勤；E-mail: ziqinxu@163.com；Tel: 029-88303484

摘　要：

以甜高粱成熟种子为外植体, 调节不同生长调节物质配比建立甜高粱离体再生体系。结果表明在MS+2.5 mg•L-1 2,4- D+0.3 mg•L-1 KT 培养基上愈伤组织的诱导率可达 77.26%; 比较不同浓度 6-BA 或 TDZ 与 NAA 配合诱导愈伤组织分化和 苗形成的情况, TDZ 的作用优于 6-BA。观察培养组织的结构变化发现, 甜高粱离体再生过程中除了体细胞胚发生途径之 外, 还伴随有器官发生途径。

关键词：甜高粱; 植株再生; 体细胞胚发生; 器官发生

收稿：2009-03-13   修定：2009-05-06

资助：国家自然科学基金(30870194)、陕西省自然科学基金(2006C103)、陕西省重点实验室科研计划(04JS07 ,08JZ70)和陕西省教育厅科研计划(05JK304, 08JK466)

In vitro Regeneration and Histological Observation in Sweet Sorghum

XU Dan, CHEN Li-Yu, XU Zi-Qin*

Provincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, China

Corresponding author: XU Zi-Qin; E-mail: ziqinxu@163.com; Tel: 029-88303484

Abstract:

In vitro regeneration system of sweet sorghum was established in the present work. Mature seeds were adopted as explants. Various combinations of plant hormones were applied for callus induction and differentiation. The callus induction rate could reach 77.26% on MS medium supplemented with 2.5 mg·L^-12, 4-D and 0.3 mg·L^-1 KT. TDZ had better effect on regeneration of plantlets than 6-BA when combined with NAA, respectively. Histological analysis revealed that both somatic embryogenesis and organogenesis existed in the process of in vitro regeneration in sweet sorghum.

Key words: sweet sorghum; plant regeneration; somatic embryogenesis; organogenesis